proteintech antibodies

[DanaiBD]

Hello everyone,
I wanted to ask whether any one has experience with proteintech antibodies. I am interested in conjugating one, but it contains (https://www.ptglab.com/products/DIO2-An ... 3-1-Ig.htm) 50% glycerol (pH 7.3) and I am unsure whether that could cause an issue.
Thank you in advance for your help

[mleipold]

Glycerol will not interfere with the chemistry of the conjugation.

However, some people have reported that the viscosity of high-percentage glycerol solutions makes it harder to mix or recover from the spin filter. In most cases, doing additional buffer washes to wash out the glycerol is good enough to address this issue.


Mike

[marthabrainard1]

Hi Dana,

I just conjugated an antibody that was stored in 50% glycerol last week. Prior to the conjugation, I mixed the antibody 1-1 with PBS and spun it down (12,000g 10 minutes) in a 30 kDa filter, it worked nicely (as Mike already mentioned!)

[DanaiBD]

Thank you very much everyone