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proteintech antibodies

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It is fine to promote your company's reagents. Just make sure they are relevant to CyTOF, and do so in moderation and style :-)
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DanaiBD

Participant

Posts: 7

Joined: Mon Oct 24, 2022 12:28 pm

Post Mon Oct 24, 2022 3:14 pm

proteintech antibodies

Hello everyone,
I wanted to ask whether any one has experience with proteintech antibodies. I am interested in conjugating one, but it contains (https://www.ptglab.com/products/DIO2-An ... 3-1-Ig.htm) 50% glycerol (pH 7.3) and I am unsure whether that could cause an issue.
Thank you in advance for your help :)
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mleipold

Guru

Posts: 5442

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Tue Oct 25, 2022 2:50 pm

Re: proteintech antibodies

Glycerol will not interfere with the chemistry of the conjugation.

However, some people have reported that the viscosity of high-percentage glycerol solutions makes it harder to mix or recover from the spin filter. In most cases, doing additional buffer washes to wash out the glycerol is good enough to address this issue.


Mike
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marthabrainard1

Contributor

Posts: 20

Joined: Wed Aug 25, 2021 2:10 pm

Post Tue Oct 25, 2022 3:03 pm

Re: proteintech antibodies

Hi Dana,

I just conjugated an antibody that was stored in 50% glycerol last week. Prior to the conjugation, I mixed the antibody 1-1 with PBS and spun it down (12,000g 10 minutes) in a 30 kDa filter, it worked nicely (as Mike already mentioned!)
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DanaiBD

Participant

Posts: 7

Joined: Mon Oct 24, 2022 12:28 pm

Post Tue Oct 25, 2022 3:23 pm

Re: proteintech antibodies

Thank you very much everyone :D

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