CyTOForum

Hi!

I am planning to perform polymer conjugation with Ag(I) and I am therefore wondering if someone has any experience conjugating silver or other monovalent ions? Would the Maxpar MCP9 or X8 antibody labeling kits be able to perform successful conjugation?

Thanks,
Nicolas

Hi Nicolas,

Short answer: I've never tried putting silver/Ag(I) into MCP9 or X8.

Longer answer: I think there's only a low probability that Ag(I) would stably interact with the chelators in either polymer. We know that X8/DN3 use DTPA chelators. I don't think Fluidigm/SBio has formally declared what chelator is used in MCP9, but since it binds Cadmium and another paper (viewtopic.php?f=10&t=2549 ) used MCP9 to bind Pd2+, there's a high likelihood that MCP9's chelator is EDTA or something very similar.

Dojindo has a Metal+Chelator stability constant sheet: https://www.dojindo.eu.com/images/Produ ... stants.pdf

Ag(I) + DTPA = 8.70
Ag(I) + EDTA = 7.32

For comparison:
Tb3+ + DTPA = 22.71
Pd2+ + EDTA = 26.40
Cd2+ + EDTA = 16.46

So, Ag(I) is *MUCH* less stable in DTPA or EDTA.


Mike