Cisplatin quench
We are trying to switch up our protocol and with the changes, it makes the most sense for us to perform viability staining in a plate. However, we can't quench cisplatin at 5x or 10x this way. Has anyone used another reagent to quench cisplatin or stained in deep well plates? Would 1-2mls be enough to quench the reaction? We currently quench at 5 or 10x with CSB.
Happy Thursday,
Martha