Mon Oct 18, 2021 10:26 pm by mleipold
Hi Martha,
When this paper was still a preprint, Felix spoke at a Stanford CyTOF meeting. When I asked him in email after the meeting, he confirmed that:
"Yes, I treated it exactly as the cisplatin [in Fienberg et al], so resuspend to 100mM in DMSO and actually we also "pre-conditioned" both at 37C for 2 days (I took this from Sean but did not do direct comparisons if this is necessary). Afterwards, I froze it at -20C (it can be refrozen at least a few times) and I made pre-dilutions in PBS (1:200) which actually seemed to work great for at least 2 weeks but I did not thoroughly compare longer term storage."
So, make a 100mM stock of DCED in DMSO, condition it, and then freeze at -20C. Yes, I would also agree that repeated freezing and thawing probably isn't a good idea, so single-use aliquots are probably best. From the paper, the 500nM final concentration is a 200x dil of that 100mM stock; the staining was done in 1mL, so a single-use aliquot at 5uL of 100mM stock shouldn't be terrible to pipet....unless you do the entire 1g bottle at once (at MW 237.42 g/mol, 1g would make 42mL @ 100mM, if my math is right).
Mike