CD45RA staining issues for smart tube fixed cells
This is Avi from UNC-Chapel Hill. As part of our core services we have been trying to optimize a panel for our clients on SMART tube fixed whole blood cells and get it going on clinical samples. However, we are encountering a CD45RA staining issue.
We have been using a CD45RA HI100 clone from Fluidigm. Thermofisher also mentions that HI100 is resistant to fixation (https://www.thermofisher.com/us/en/home ... tions.html).
1. Has anyone encountered such issues with CD45RA staining on post fixation cells ?
2. If so, which clone works better for post fixation staining of whole blood cells ?
Thanks for your inputs in advance
Regards
Avinash