Diluting antibodies in stabilization buffer
Are there any concerns about over diluting antibodies in antibody stabilization buffer? I typically dilute to 0.2mg/ml until titration and then further dilute so that they can be used at ~1uL/test. In some cases, this second phase of dilution is quite significant (>1mL). Would this be expected to have any impact on long term stability?
Icahn School of Medicine at Mount Sinai, NYC