Number of Metals Per Antibody
Posted: Thu Feb 27, 2020 10:43 pm
Hi all,
Do you determine the number of metal atoms per antibody for custom conjugated antibodies? If so what procedure do you use and what is the typical number of metal atoms per antibody that you observe? Literature and Fluidigm says that there ought to be 100-200 metal atoms per antibody but, I am observing a wide range depending on the antibody (as low as 30 and as high as 105). Does anyone have any input on this? Thank you!
Do you determine the number of metal atoms per antibody for custom conjugated antibodies? If so what procedure do you use and what is the typical number of metal atoms per antibody that you observe? Literature and Fluidigm says that there ought to be 100-200 metal atoms per antibody but, I am observing a wide range depending on the antibody (as low as 30 and as high as 105). Does anyone have any input on this? Thank you!