Quantifying Matrigel in CyTOF
Posted: Mon Sep 02, 2019 4:15 pm
Hi all!
Hope everyone is well. I've got a question about the above subject.
I'm trying to measure the amount of matrigel in our assay. One way we're thinking of doing this is by chelating an NHS-ester probe to a metal, then binding that probe onto matrigel, wash out the wells a couple times to remove all unbound material, then removing the matrigel from the wells and running that on the CyTOF. My questions are:
1) Is this feasible? i.e. can it work or will there just be huge tyre tracks on the rainplot?
2) Can the above be run in solution mode of the CyTOF? What is the readout of solution mode and how does it actually work? Do you read-out events?
3) Otherwise, if 1) and 2) isn't feasible, does anyone know of any beads which bind to proteins? So we can add those beads into the solution of matrigel and then all of the matrigel would stick to the beads. We would then run those beads on the CyTOF in event mode and we would see the events coming through of those matrigel droplets which have absorbed the NHS-ester probe.
It would be great if anyone has any thoughts on this!
Many thanks,
Jahangir
Hope everyone is well. I've got a question about the above subject.
I'm trying to measure the amount of matrigel in our assay. One way we're thinking of doing this is by chelating an NHS-ester probe to a metal, then binding that probe onto matrigel, wash out the wells a couple times to remove all unbound material, then removing the matrigel from the wells and running that on the CyTOF. My questions are:
1) Is this feasible? i.e. can it work or will there just be huge tyre tracks on the rainplot?
2) Can the above be run in solution mode of the CyTOF? What is the readout of solution mode and how does it actually work? Do you read-out events?
3) Otherwise, if 1) and 2) isn't feasible, does anyone know of any beads which bind to proteins? So we can add those beads into the solution of matrigel and then all of the matrigel would stick to the beads. We would then run those beads on the CyTOF in event mode and we would see the events coming through of those matrigel droplets which have absorbed the NHS-ester probe.
It would be great if anyone has any thoughts on this!
Many thanks,
Jahangir