Reagents differences
Hi Guys,
I have a new marker on my CyTOF panel that I believe is expressed "everywhere". It is cytoplasmic, secreted and nuclear.
I was wondering which protocol I should use for that.
My idea was to use the Maxpar Cytoplasmic/Secreted Antigen Staining with Fresh fix protocol from Fluidigm.
For that, I should use Maxpar Fix I Buffer (5x) cat#201065 and Maxpar Perm-S Buffer cat# 201066
I was wondering if I could use MAxpar solutions from Fluidigm Barcode for this.
In this case instead of Maxpar Perm-S Buffer I will be using Maxpar 10X Barcode Perm.
The Maxpar Fix I Buffer looks the same to me but they have different catalog numbers. The barcode kit FIX I solution is #S00115. Does anyone knows if they are the same?
Thanks very much!
All the Best!
Thais
I have a new marker on my CyTOF panel that I believe is expressed "everywhere". It is cytoplasmic, secreted and nuclear.
I was wondering which protocol I should use for that.
My idea was to use the Maxpar Cytoplasmic/Secreted Antigen Staining with Fresh fix protocol from Fluidigm.
For that, I should use Maxpar Fix I Buffer (5x) cat#201065 and Maxpar Perm-S Buffer cat# 201066
I was wondering if I could use MAxpar solutions from Fluidigm Barcode for this.
In this case instead of Maxpar Perm-S Buffer I will be using Maxpar 10X Barcode Perm.
The Maxpar Fix I Buffer looks the same to me but they have different catalog numbers. The barcode kit FIX I solution is #S00115. Does anyone knows if they are the same?
Thanks very much!
All the Best!
Thais