CCR7 erased
Hello,
We seem to have a problem with the CCR7-159Tb antibody from Fluidigm (Clone G043H7) - although the antibody should be enough for 50 tests, 1ul is not enough.
Our protocol is as follows: Barcoding, EC staining, fix, Methanol perforation, IC staining, fix, Ir staining.
All the other antibodies work well, and only the CCR7 is not. We have done a calibration experiment where we found only using 5ul of the antibody worked. Using different vendors with 5ul volume of the antibody did not yield a good result...
Fluidigm claims the problem is with the barcoding fix.
We need to have both the barcoding and methanol perm for pFlow, and at the same time CCR7 is imporatant to our panel.
Did anyone run into a similar problem? If so, what was your solution?
Thanks.
We seem to have a problem with the CCR7-159Tb antibody from Fluidigm (Clone G043H7) - although the antibody should be enough for 50 tests, 1ul is not enough.
Our protocol is as follows: Barcoding, EC staining, fix, Methanol perforation, IC staining, fix, Ir staining.
All the other antibodies work well, and only the CCR7 is not. We have done a calibration experiment where we found only using 5ul of the antibody worked. Using different vendors with 5ul volume of the antibody did not yield a good result...
Fluidigm claims the problem is with the barcoding fix.
We need to have both the barcoding and methanol perm for pFlow, and at the same time CCR7 is imporatant to our panel.
Did anyone run into a similar problem? If so, what was your solution?
Thanks.