Magnetic beads selection - ?interference
we are about to run a large collaborative project where I will be given positively selected human lymphocytes for CyTOF. I am worried that the magnetic beads might interfere with other channels. I see that people advice to use negative selection if possible, but that is unfortunately not an option for me.
a) Do you know if Miltenyi magnetic beads used in selection kits cause problems? If yes, is this interference with one particular channel or multiple channels?
b) Do you know of any other company which might be selling CyTOF friendly selection kits (based on magnetic beads)?
c) Some people are suggesting to strip off the beads from the cells before running CyTOF. Do you have any protocols how this is done? Does such treatment affect other surface markers?
Many thanks for your comments to any of my questions!
Best regards,
Ondrej