Hi Cisplatin-geeks ^^
I was on holidays so I answer quiet late... Sorry.
I did follow the protocol from the Mei et al, Cytometry A, 2015 paper, without any pretreatment.
For storage, basically, what I was told by Petr Vasiliev from Buyisotopes was that Cisplatin is very sensitive to heat (and light !), and that freezing/thawing should be totally avoided !!! Consequently, I did aliquots like you Jahangir, except that I kept the Cisplatins at 10mM and made aliquots of 2µL, as for 1 AB conjugation reaction, and store them in -20°C. Quiet a long task as we got 1.5 to 2 ml
To be continued with the 194/195 Cisplatins
Olivier
(Lots of discussions to read after 3 weeks !)