CyTOF Sample Tubes
Hey everyone,
Our lab is just getting started on CyTOF and need to buy some disposables for staining, etc. When I did the staining protocol at our FACS core with the technician, we were using silicon-coated epi tubes (Fisher #02-681-320) for staining, however, I didn't quite like that the syringe that we need to use to load the CyTOF didn't reach all of the cell suspension in the epi tube.
Has anyone found a better solution for this? Perhaps a different type of epi tube or a simply a technique change that we aren't realizing?
Nick
Our lab is just getting started on CyTOF and need to buy some disposables for staining, etc. When I did the staining protocol at our FACS core with the technician, we were using silicon-coated epi tubes (Fisher #02-681-320) for staining, however, I didn't quite like that the syringe that we need to use to load the CyTOF didn't reach all of the cell suspension in the epi tube.
Has anyone found a better solution for this? Perhaps a different type of epi tube or a simply a technique change that we aren't realizing?
Nick