problem with Fluidigm Pd barcoding
We recently are experiencing problems with the Fluidigm Pd barcode staining of PBMC's. I initially noticed that intensity on the rain plot was not great, and when I debarcoded the data I found that we had very low yield. Normally we recover over 200,000 events and with this new lot of barcodes, I recovered from 20-30,000 events. I compared old barcodes to new barcodes and found that I could recover my usual 200,000 events with the old, but the new was only 32,000. Fluidigm sent us a new lot and we saw the same thing. I am wondering if others are experiencing this?? I've been using the Cytof software for debarcoding.
Thanks,
Diane