Overnight Background Increase
I've stained PBMC with surface markers. Then I washed and fixed with 1.6% paraformaldehyde. The 16% PFA was fresh from a break open vial, and diluted with Ca++/Mg++ free PBS. Half of the sample was collected on the Cytof, and the other half was placed at 4C for collection the next day. In comparing the data, the only differences I saw were in the background. The overnight sample had increased nonsense populations like CD3+ and CD20+. Is this to be expected? Is there a way to avoid it?
I've attempted to attach a pdf.
Ian