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Impact of cisplatin on phospho

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Kjwaller

Participant

Posts: 11

Joined: Sat Aug 27, 2016 6:56 am

Post Fri Oct 07, 2016 4:28 pm

Impact of cisplatin on phospho

Hi,

I am about to begin my main set of cyTOF experiments and I was wondering whether anyone had any experience using cisplatin for viability then doing phospho flow? I know on the fluidigm website it says it doesn't interfere with PMA but they don't make any claims for anything else. Has anyone found it to interfere with their signal?

Thanks,
Kate
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mleipold

Guru

Posts: 1778

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Fri Oct 07, 2016 4:51 pm

Re: Impact of cisplatin on phospho

Hi Kate,

As stated in the Fluidigm cisplatin TDS, the primary literature for cisplatin as a viability indicator is the 2012 Fienberg et al paper:

viewtopic.php?f=10&t=14&p=14&hilit=fienberg#p14

In it, they demonstrate that cisplatin at the levels used for viability staining do not affect pervandate signalling (Fig 4), nor DNA Damage Response or Apoptosis out to at least 6 hr (Fig 5). Even taking into account post-cisplatin wash steps immediately prior to stim, your cells should be fixed prior to any effect on signalling.


Mike

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