Do we have some way to increase the cell recovery efficiency
Posted: Mon Sep 26, 2016 2:41 pm
Hello! just saw some papers about Circulating Tumor Cells, quite few in blood, probably not possible be analyzed by CyTOF, because the cell recovery efficiency in CyTOF is low. Just wondering whether have some way to increase cell recovery efficiency in CyTOF?
As previously discussed, ''CyTOF machine itself has a cell transmission efficiency of only 20-30%. In other words, if you give it 1 million cells, the MAX you can usually expect to recover would be 200-300K cells as "Live Intact Singlets" (not "Found Cells", which includes debris and other background) . This is an inherent design issue to the CyTOF: cells stick to the sample loop, nebulizer tubing, nebulizer, and are lost to the spray chamber''.
Do we have some way to increase this cell recovery efficiency, from experiment, engineer or theory perspective?
As previously discussed, ''CyTOF machine itself has a cell transmission efficiency of only 20-30%. In other words, if you give it 1 million cells, the MAX you can usually expect to recover would be 200-300K cells as "Live Intact Singlets" (not "Found Cells", which includes debris and other background) . This is an inherent design issue to the CyTOF: cells stick to the sample loop, nebulizer tubing, nebulizer, and are lost to the spray chamber''.
Do we have some way to increase this cell recovery efficiency, from experiment, engineer or theory perspective?