Labeling an antibody preconjugated to PE or FITC
Posted: Mon Jun 06, 2016 1:33 pm
Hi all,
At our group we're labeling the antibodies ourselves. Nearly all antibodies are available in purified form, but for one it is only available already conjugated to either PE or FITC. Does anyone have any experience with conjugating a metal isotope to an antibody+fluophore? We reckon once the labeled cell hits the plasma torch it shouldn't matter for your measurement but we're not sure about the conjugation process.
Any feedback is much appreciated!
Greetings,
Jan
At our group we're labeling the antibodies ourselves. Nearly all antibodies are available in purified form, but for one it is only available already conjugated to either PE or FITC. Does anyone have any experience with conjugating a metal isotope to an antibody+fluophore? We reckon once the labeled cell hits the plasma torch it shouldn't matter for your measurement but we're not sure about the conjugation process.
Any feedback is much appreciated!
Greetings,
Jan