Iodine Contamination
I am new to CyTOF and I have been experiencing Iodine contamination in my samples which sometime prevent me from running them through the CyTOF machine!!
I have been using Maxpar PBS (From Fluidigm), Maxpar Cell Staining buffer (From Fluidigm), Maxpar Water (From Fluidigm), MACS buffer, Myelin Removal Beads II (from Miltenyi Biotec), Ficoll (From Sigma Aldrich), And the Antibodies are all from Fluidigm
I mash the tumor tissues against the strainer suing the plastic part of the syringe
I do the tumor removal and dissociation on my bench but the myelin removal under the hood
I would appreciate your suggestions and insights regarding this issue!