Blocked nebuliser / nebulizer
Posted: Fri May 17, 2019 8:03 am
Hi all,
Does anyone have advice on how to clear a blocked / clogged nebuliser?
We were running what looked like (by eye) lovely clear samples (mouse splenocytes) filtered and diluted to achieve ~ 300 events/sec.
I managed to clear the clogs a couple of times by flushing with 10% decon 90 (as per Fluidigm instructions) and carry on running.
However, every ~10 mins the freshly cleaned one would clog and now both nebulisers are still blocked after soaking overnight in 10% decon 90.
I cannot flush any liquid through from the back (i.e. the sample input end).
I can flush liquid through the gas input side.
For the moment, I'm just leaving them to soak in Decon 90 until I find a way to clear them, or buy new ones.
I've read about a mysterious (to me) Pearl Cleaner, but unsure exactly what it does?
Does anyone have advice on how to clear a blocked / clogged nebuliser?
We were running what looked like (by eye) lovely clear samples (mouse splenocytes) filtered and diluted to achieve ~ 300 events/sec.
I managed to clear the clogs a couple of times by flushing with 10% decon 90 (as per Fluidigm instructions) and carry on running.
However, every ~10 mins the freshly cleaned one would clog and now both nebulisers are still blocked after soaking overnight in 10% decon 90.
I cannot flush any liquid through from the back (i.e. the sample input end).
I can flush liquid through the gas input side.
For the moment, I'm just leaving them to soak in Decon 90 until I find a way to clear them, or buy new ones.
I've read about a mysterious (to me) Pearl Cleaner, but unsure exactly what it does?