Post Mon May 30, 2022 5:58 pm

MIBI - Webinar - GenomeWeb, June 1st 2022

Proteomic Characterization of the Tissue Microenvironment with Multiplexed Ion Beam Imaging (MIBI)

Dear all,
On June 1st at 12:00 PM EDT Dr. Tomer-Meir Salame will be giving a 20 min webinar via GenomeWeb about MIBI:
Proteomic Characterization of the Tissue Microenvironment with Multiplexed Ion Beam Imaging (MIBI)
Registration is free:
https://event.on24.com/wcc/r/3759244/5A ... athspeaker


Summary
Characterization of the cellular composition and spatial organization of the tissue microenvironment has been limited by the techniques available to image the necessary number of biomarkers for broad phenotyping at a subcellular scale.

Multiplexed ion beam imaging (MIBI) combines time-of-flight secondary ion mass spectrometry (ToF-SIMS) with metal-labeled conventional antibodies to image over 40 biomarkers in situ in a single scan at subcellular spatial resolution.

In this webinar, Tomer-Meir Salame, staff scientist in the life sciences core facilities of the Weizmann Institute of Science, will present the MIBI platform and explain its workflow from the perspective of a core facility, providing insight into how this imaging method can be used for studies that require high-dimensional and deep characterization of tissue microenvironments.

MIBI enables quantitative, full periodic table coverage across a five-log dynamic range, imaging all labeled antibodies simultaneously with fields of view up to 800 μm × 800 μm at resolutions down to 350 nm and sensitivities approaching single-antibody detection. MIBI analysis can be performed on FFPE, fresh, or frozen tissue.

Salame will illustrate the platform’s capabilities through a cohort of clinical tissue sections taken from a case study focusing on the characterization of the microenvironment of sentinel lymph nodes in relation to melanoma metastasis.


Speakers
Tomer-Meir Salame has served as a staff scientist in the flow cytometry unit of the Life Sciences Core Facilities at the Weizmann Institute of Science in Rehovot, Israel since 2017. He joined the Weizmann Institute in 2014 after completing his doctorate in biotechnology, with distinction, from the Hebrew University. He has deep expertise in mass spectrometry-related applications in life science and has won over 30 awards and scholarships, including the Wolf Fellowship, The Robert H. Smith Vision Prize, and the Rector’s Award.

At the Weizmann Institute, he has led the implementation of mass cytometry technologies — both CyTOF and MIBI — for research programs there. He has collaborated on over 30 multidisciplinary projects and over 40 peer-reviewed publications. He also teaches cytometry coursework.

Salame is an active member of the International Society for Advancement of Cytometry (ISAC) and serves as a reviewer on the ISAC SRL (Shared Resource Laboratories) Recognition Program Sub-Committee.
Attachments
Tomer_MIBI_01June2022.JPG
Tomer_MIBI_01June2022.JPG (208.97 KiB) Viewed 264 times
Dr. Tomer-Meir Salame
Head, Mass Cytometry Unit
Life Sciences Core Facilities
Weizmann Institute of Science
E-mail: tomer-meir.salame@weizmann.ac.il
http://wws.weizmann.ac.il/Biological_Se ... etry-about