How to prevent "sticky" cells clogging machine
Do you have some protocol or method can prevent "sticky" cells forming aggregation?
Is there any other reason can cause this issue?
Much appreciate it!
Xiao
Contributor
Posts: 47
Joined: Mon Oct 05, 2015 9:48 am
Location: European Genomic Institute for Diabetes, Universitity of Lille, Institut Pasteur de Lille, France
RDevine wrote:Hi Xiao,
We've been experimenting with an EDTA and DNAse solution but we haven't really perfected it. We designed this to be added in at any time, as soon as clumping is noticeable but when we set up an experiment to test out a titration of EDTA and DNAse solution cells that were reliably clumping stop clumping all of a sudden. We've experimented with 10U/ml DNase and 5 mM EDTA solution thats added to the sample and left in 37C incubator for 20 minutes after the Ir incubation. We've also tried just EDTA and we've had some success but we haven't really been able to experiment with it thoroughly.
Ray
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