Skimmer/Reducer Cone Issue
We have a CyTOF 1 and have gotten replacement skimmer/reducer cones that don't seem to work. Instead of getting good separation of our singlets and doublets, we get a stream. I was told that Fluidigm does not make these cones but they will test them before sending them out so... we asked for a tested one and it still had poor separation. Has anyone else had problems like this? Did you get your cones from Fluidigm and if so, did you request them to be tested. Also, how long does it take to get a cone from them? Any information would be helpful.
Thanks!
Thanks!