Argon gas intensity?
Dear Helios User,
We recently upgraded CyTOF2 to Helios. The standard protocol require that argon gas intensity has to be above 5 millions before tuning. But I usually can't get such high gas intensity although every connection parts are tight enough. sometimes after full protocol tuning, it can be over 5 millions, but sometimes not (Tuning can pass with Tb159 at about 1000K).
any suggestion to this gas issue? does this low gas intensity will affect data acquisition? signal intensity? Thanks!
Liyun
We recently upgraded CyTOF2 to Helios. The standard protocol require that argon gas intensity has to be above 5 millions before tuning. But I usually can't get such high gas intensity although every connection parts are tight enough. sometimes after full protocol tuning, it can be over 5 millions, but sometimes not (Tuning can pass with Tb159 at about 1000K).
any suggestion to this gas issue? does this low gas intensity will affect data acquisition? signal intensity? Thanks!
Liyun