invariant NK cells
I'm new to the Cytof so please bear with me. I'm analysing PBMCs post-transplant but currently I'm trying to optimise my panel on healthy samples from frozen.
I have an invariant NK cell marker (TCRVa24Ja18) that I am using from Fluidigm. I have used a protocol that combines the cell surface followed by the nuclear staining protocol (I have other markers too!) and used 1uL of the antibody.
I realise the population is very small (?0.2% of lymphocytes) but I have approx 700,000 cells, so I would have expected to find some positive cells, but it doesn't look like it has worked.
I wondered if anyone else has any experience of using this marker? Have you had to titrate up a lot? Any good positive control cells I could use?
Many thanks,
James