different metal channels, same mass
Hi guys,
Three questions about my run!
When running CyTOF acquisition, we had left two of the same mass channels open (CE142 and Nd142). When I'm looking at the data, I was wondering if there is increased potential for spillover into the +1 and +16 channels when two masses of the same weight are open?
Second, When looking at my nuclear stain, I think I am using too much, but I just wanted to clarify. If there isn't excess staining (I still see clean cell events), is there a problem that it is this high?
Third: I have been seeing this "wandering" population that I am seeing in IgD+, CD31+ in the image attached is an issue of panel design?
Thanks in advance for all your help!
Three questions about my run!
When running CyTOF acquisition, we had left two of the same mass channels open (CE142 and Nd142). When I'm looking at the data, I was wondering if there is increased potential for spillover into the +1 and +16 channels when two masses of the same weight are open?
Second, When looking at my nuclear stain, I think I am using too much, but I just wanted to clarify. If there isn't excess staining (I still see clean cell events), is there a problem that it is this high?
Third: I have been seeing this "wandering" population that I am seeing in IgD+, CD31+ in the image attached is an issue of panel design?
Thanks in advance for all your help!