Post Mon Sep 28, 2015 4:03 pm

low signal (clog?)

Dear Fellow CyTOFers,

During QC, I have been getting very low signal when running the tuning solution (Tb159 signal is ~100,000 mean dual count) as well as very low EU151/153 mean intensity (~300). I have double checked all gas connections, possible leaks, and clogs in the nebulizer/sample loops (ie: I get a very nice spray). One thing I noticed was when I tried to manually optimize current/argon gas flow/xy position, nothing altered the signal intensity...

I'm thinking perhaps there is a partial clog in the internal loops and ran wash solution several times to no avail..do you guys have any tips on how to better deal with potential clogs? Or any other ideas on what the diagnosis might be?

Thanks so much!

Best,
Joel