CyTOF Data Normalization
Hello CyTOFER
This is Sam Menge, a researcher at SDI at the University of Minnesota Medical School. I was running CyTOF samples last week and realized after completing the run for all of the samples that I did not have the 140etc (Beads) channel in my panel on the computer application, even though I did add the bead mix to each of the samples. When I was trying to normalize the data later, the program we use would not let me normalize it as it kept saying beads were missing from the application or something similar of that nature. I was wondering if we could find some way to add the 140etc channel to the data so that we can normalize and still use it. If you have any other ideas about how I could get past this normalization issue, please let me know.
Thanks for your time and help,
Sam Menge
and
Amar Singh
This is Sam Menge, a researcher at SDI at the University of Minnesota Medical School. I was running CyTOF samples last week and realized after completing the run for all of the samples that I did not have the 140etc (Beads) channel in my panel on the computer application, even though I did add the bead mix to each of the samples. When I was trying to normalize the data later, the program we use would not let me normalize it as it kept saying beads were missing from the application or something similar of that nature. I was wondering if we could find some way to add the 140etc channel to the data so that we can normalize and still use it. If you have any other ideas about how I could get past this normalization issue, please let me know.
Thanks for your time and help,
Sam Menge
and
Amar Singh