Re: CD4+ Yield in Whole Blood
Hi Chad,
Definitely let us know the results of the standard flow run. I remember having a lot of trouble with cell recovery using polystyrene 5mL FACS tubes, especially after fixation and during the water wash a good pellet never formed. I ended up moving most of my staining to polypropylene tubes and if possible used conicals since most of the cell loss seemed to come from wash steps.
Rob
Definitely let us know the results of the standard flow run. I remember having a lot of trouble with cell recovery using polystyrene 5mL FACS tubes, especially after fixation and during the water wash a good pellet never formed. I ended up moving most of my staining to polypropylene tubes and if possible used conicals since most of the cell loss seemed to come from wash steps.
Rob