Cisplatin for cell ID
Has anyone tried using cisplatin for cell-ID in place of or at the same time as Ir191/193? I have 2 different labs attempting to use it as a means of barcoding however in both cases we have encountered what appears to be dissociation of cells. They are using natPt (Pt195) for viability, Ir to identify all cells and barcoding with Pt194, Pt198 and Rh103. The barcoded channels should always be also positive for Ir but were not always. The Ir positive cells should have always also been positive for one of the barcodes but were not. Instead we see a large number of Ir negative, Pt barcoded positive events. There also appear to be a large number (in comparison to non-barcoded samples) of events that are negative for both Pt and Ir but look like real cell events. It also appeared like there was a large number of events that were either Ir or Pt positive but negative for all of the markers. We would love to know if anyone else has seen this and has suggestions on improving the results
Thanks!
Nicole
Thanks!
Nicole