Contributor
Posts: 44
Joined: Tue May 20, 2014 2:24 pm
Location: Linkoping University, Sweden and Finnish Institute of Molecular Medicine, Finalnd
HI I really cant find L-Sca-1+Ckit+ cells in CyTOF.
I have used Miltenys Lineage depletion kit it worked well for Lin-Sca-1 and Ckit+ cells. The miltenys Lineage depletion kit contains CD11b, GR1, TER119 , CD5 , 4-7 and B220.
But I need B220 in lineage depleted cells. So we decided to make our own panel having Biotine CD11b, TER119, CD5 and GR1 from eBiosceince. optimized for best depletion but still cant find cKit and Sca-1 cells in CyTOF. purchased and tried different metal conjugated antibodies for cKit and Sca-1 but still cant find the cells. We are planning to do a real experiment if we find cKit and Sca-1 cells.
Can anyone help me with this problem.
here is the image how it looks for cKit and Sca-1 cells.