Cell loss during acquisition prep
Posted: Mon Nov 08, 2021 9:31 pm
Hi Cytoforum!
I know cell loss is expected during acquisition prep, specifically when washing with CAS, but does anyone have any suggestions to mitigate this loss? We recently have been losing more cells after our 2nd CAS wash (these cells were frozen in FBS + DMSO-no beads were added until we ran). Currently we prep cells by washing 2x in CSB and 2x in CAS (1500g, 3 min).
We were thinking of testing different spin speeds and longer centrifugation times (I know fluidigm recommends 800g for 5 min), but I wanted to see if anyone else has tested this.
I know cell loss is expected during acquisition prep, specifically when washing with CAS, but does anyone have any suggestions to mitigate this loss? We recently have been losing more cells after our 2nd CAS wash (these cells were frozen in FBS + DMSO-no beads were added until we ran). Currently we prep cells by washing 2x in CSB and 2x in CAS (1500g, 3 min).
We were thinking of testing different spin speeds and longer centrifugation times (I know fluidigm recommends 800g for 5 min), but I wanted to see if anyone else has tested this.