Low Tb counts with Auto tuning
We have recently been trying to figure out an issue with auto tuning our CyTOF2. What we are seeing is a low Tb dual counts, with reasonable oxide ratios typically less than 2%, good resolution (450 or 412), typical gas flow rates, cone currents between 3-4.5 after a deep clean of all parts. We have replaced the nebulizer, sampler cone, sample capillary line, one at a time, with new parts and have not found any to be helpful. Detector voltage relatively stable but creeping up as normal. We started using a new bottle of tuning solution around the time, +/- a few days, this pattern started.
Any thoughts or suggestions would be great to hear!!
Emily