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Non-specific binding for Intracellular Targets

PostPosted: Wed Apr 07, 2021 10:31 pm
by nameerabaig
Dear Forum,

Has anyone experienced significant non-specific binding for intracellular targets? I work with different cell types such as fibroblasts in addition to immune cells and notice that there is sometimes a significant contribution from non-specific binding for intracellular targets. I have read literature where heparin was used to block non-specific binding for eosinophils. Can this treatment be used for other cell types that are not blood cells? Any feedback will be very helpful. Thank you very much!

Re: Non-specific binding for Intracellular Targets

PostPosted: Fri Apr 09, 2021 10:16 pm
by mleipold
Hi Nameera,

I would recommend that you just try staining +/- heparin to see whether it makes a difference in your fibroblasts. While the reduction in background been mostly clearly demonstrated with eosinophils, I don't think it would be completely limited to just that cell type.


Mike

Re: Non-specific binding for Intracellular Targets

PostPosted: Tue Jun 01, 2021 2:59 pm
by nameerabaig
Thank you all!