Non-specific binding for Intracellular Targets
Posted: Wed Apr 07, 2021 10:31 pm
Dear Forum,
Has anyone experienced significant non-specific binding for intracellular targets? I work with different cell types such as fibroblasts in addition to immune cells and notice that there is sometimes a significant contribution from non-specific binding for intracellular targets. I have read literature where heparin was used to block non-specific binding for eosinophils. Can this treatment be used for other cell types that are not blood cells? Any feedback will be very helpful. Thank you very much!
Has anyone experienced significant non-specific binding for intracellular targets? I work with different cell types such as fibroblasts in addition to immune cells and notice that there is sometimes a significant contribution from non-specific binding for intracellular targets. I have read literature where heparin was used to block non-specific binding for eosinophils. Can this treatment be used for other cell types that are not blood cells? Any feedback will be very helpful. Thank you very much!