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Non-specific binding for Intracellular Targets

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nameerabaig

Participant

Posts: 7

Joined: Wed Nov 20, 2019 5:20 pm

Post Wed Apr 07, 2021 10:31 pm

Non-specific binding for Intracellular Targets

Dear Forum,

Has anyone experienced significant non-specific binding for intracellular targets? I work with different cell types such as fibroblasts in addition to immune cells and notice that there is sometimes a significant contribution from non-specific binding for intracellular targets. I have read literature where heparin was used to block non-specific binding for eosinophils. Can this treatment be used for other cell types that are not blood cells? Any feedback will be very helpful. Thank you very much!
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mleipold

Guru

Posts: 5695

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Fri Apr 09, 2021 10:16 pm

Re: Non-specific binding for Intracellular Targets

Hi Nameera,

I would recommend that you just try staining +/- heparin to see whether it makes a difference in your fibroblasts. While the reduction in background been mostly clearly demonstrated with eosinophils, I don't think it would be completely limited to just that cell type.


Mike
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nameerabaig

Participant

Posts: 7

Joined: Wed Nov 20, 2019 5:20 pm

Post Tue Jun 01, 2021 2:59 pm

Re: Non-specific binding for Intracellular Targets

Thank you all!

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