Severe 209-Bismuth Background Contamination
Dear CytoFers,
I hope everyone is well.
My query is about the recent background contamination in the 209-Bi channel that my lab and I are currently observing. Please see the attached file below:
Here is a list of information that may be of benefit to you:
- This has only started to occur within the last month or so. Before this, the background in the 209-Bi was not bad whatsoever.
- The attached file has no antibody used in that channel.
- The data that is shown in the attached file is DNA+.
- There is very little to no background contamination in the 208-Pb channel. So there is no spillover from that channel. 209 - 16 = 193.
- We are using the Supersampler (with the helios system) instead of the PSI.
- I've tested all the buffers and equipment and consumables in my lab and there is no 209-Bi contamination coming from any of them.
- I've thoroughly cleaned the different parts of the SuperSampler and when running MaxPar water by itself thereafter, there isn't any 209-Bi contamination whatsoever.
So my question is, where on earth is this 209-Bi background contamination coming from? It's a great channel that we've used for a long time and would be a huge shame if we lost it.
Best regards to everyone,
Jahangir Sufi
I hope everyone is well.
My query is about the recent background contamination in the 209-Bi channel that my lab and I are currently observing. Please see the attached file below:
Here is a list of information that may be of benefit to you:
- This has only started to occur within the last month or so. Before this, the background in the 209-Bi was not bad whatsoever.
- The attached file has no antibody used in that channel.
- The data that is shown in the attached file is DNA+.
- There is very little to no background contamination in the 208-Pb channel. So there is no spillover from that channel. 209 - 16 = 193.
- We are using the Supersampler (with the helios system) instead of the PSI.
- I've tested all the buffers and equipment and consumables in my lab and there is no 209-Bi contamination coming from any of them.
- I've thoroughly cleaned the different parts of the SuperSampler and when running MaxPar water by itself thereafter, there isn't any 209-Bi contamination whatsoever.
So my question is, where on earth is this 209-Bi background contamination coming from? It's a great channel that we've used for a long time and would be a huge shame if we lost it.
Best regards to everyone,
Jahangir Sufi