Cisplatin Staining Problem
I have run into an issue with my sample analysis when trying to gate for live cells. After staining with the cisplatin live/dead from fluidigm, all of my cells end up being 195pt+ as if they are all dead?
I am running pediatric blood samples. My samples are frozen at -80 in PROT-1 stabilizer. I thaw them over ice. I have run a test on a fresh sample which stained without issue. I used the same lysis buffer on both (ACK Lysis buffer). I perform a Trypan blue cell count prior to staining and just prior to running the samples and I have live (or intact) cells at both steps by that stain.
I'm wondering if anyone else has encountered this problem or if anyone might be able to offer a solution or resources that can help me figure out what is going on.
Thanks!