Varying intensity in some channels
I have problem with data interpretation.
In my experiemnt I had control mice and treated mice.
I noticed that intensity of some channels (channel 166Er used for EpCam) for 2 of control mice was way stronger (more than 10 folds) when compared to rest of samples.
Surprisingly also another marker (functional marker) in channel 169Tm is very strong in these 2 samples.
Based on intensity of marker in 169Tm I am quantifying one of most important readout. However I am wondering whether my readout in 169Tm is simply affected by change in intensity of 166Er?? How to normalize it?
I normalized data with Fluidigm software.
I would be extremely happy for suggestions, and help.
best wishes,
julia