Thu Sep 04, 2014 8:44 pm by ChantelMcS
Cytof gating is perplexing me. In flow, I’d make gates based on population breaks and if that was not clear/obvious, I’d make an FMO. With my cytof data, I have 3 different B-cell markers that are not clear. Does it make sense (to save on ab’s etc) to make one control that would be the same as my panel minus the 3 challenging abs or must I make a “metals minus one”? Based on DVS’s chart, the 3 troubling markers do not contribute to each other.
One more question: When making “metals-minus” kind of controls, do we need to include ab-metal (that based on DVS cross talk chart) cannot contribute to the signal in question?
Thank you