Reg: Maxpar Direct Immune Profile Assay 30 Markers
Dear CyTOF people,
Has any one used Maxpar Direct Immune Profile Assay 30 Markers kit?. Soon planing to stain the cells using this kit but I have a question before staining. The protocol provided by the fluidigm specifically for this kit: after surface staining fixation with 1.6% PFA is included before adding intercalator in Fix/Perm buffer. my question is do I have to fixation step before adding Ir in Fix/Perm buffer? I was thinking adding Fix/Perm buffer is enough to fix the cells both at the surface and intercellular?
Sincerely
Komal
Has any one used Maxpar Direct Immune Profile Assay 30 Markers kit?. Soon planing to stain the cells using this kit but I have a question before staining. The protocol provided by the fluidigm specifically for this kit: after surface staining fixation with 1.6% PFA is included before adding intercalator in Fix/Perm buffer. my question is do I have to fixation step before adding Ir in Fix/Perm buffer? I was thinking adding Fix/Perm buffer is enough to fix the cells both at the surface and intercellular?
Sincerely
Komal