Whole blood staining
Hi CyTOFers!
Happy New Year 2020.
I am planning to do whole blood staining with a custom made 36 marker panel. Instead of 103Rh I plan to use 198-Cisplatin because I already have cisplatin in my lab.
Question - When should I plan to stain the cells with Cisplatin live/dead ?
A. Heparin block > Antibody cocktail staining > Lyse > Cisplatin live/dead > Fix.
B. Heparin block > Cisplatin live/dead > Antibody cocktail staining > Lyse > Fix.
C. Heparin block > Antibody cocktail + Cisplatin live/dead staining > Lyse > Fix.
Thanks
Avi
Happy New Year 2020.
I am planning to do whole blood staining with a custom made 36 marker panel. Instead of 103Rh I plan to use 198-Cisplatin because I already have cisplatin in my lab.
Question - When should I plan to stain the cells with Cisplatin live/dead ?
A. Heparin block > Antibody cocktail staining > Lyse > Cisplatin live/dead > Fix.
B. Heparin block > Cisplatin live/dead > Antibody cocktail staining > Lyse > Fix.
C. Heparin block > Antibody cocktail + Cisplatin live/dead staining > Lyse > Fix.
Thanks
Avi