CD signal decreasing over time...
Hope everyone is well.
So, my colleague is running some human gdT-Cells. The attached screenshots are all ungated biaxials for:
- CD45 (HI30) against time
- CD25 (2A3) against time
- CD44 (IM7) against time
But as you can see, the signal slightly decreases over time... has anyone got any suggestions why this is occurring?
It's only happening with these specific antibodies and not any intracellular antibodies.
Many thanks,
Jahangir