Post Thu Jun 03, 2021 7:03 am

Re: Progressive increase in signal background

Hi Mike and all,

Usually the filter does not need to be replaced, especially if running PBMCs etc.
Since we are working in a core facility environment, we replace it as part of our daily startup routine SOP (and the filter is cheap).
It is also possible to declog (i.e. backflush) with the filter connected, and to run the tuning and wash solutions through them with no issues.

Yes, these filters are "clean". Validation of the rain plot in TOF mode (and recording of DIW in solution mode) are also part of our daily QC.

Most welcome,

P.S. the sample line does not need to be disassembled from the PSI to mount/replace the filter, though small fingers are an advantage for access from the bottom.
Dr. Tomer-Meir Salame
Head, Mass Cytometry Unit
Life Sciences Core Facilities
Weizmann Institute of Science
E-mail: ... etry-about