Event Length settings in CyTOF/Flowjo softwares
We recently acquired CyTOF2 in Cape Town. After performing a standard experiment of PBMC stimulation with mitogen, the Event_length parameter on flowjo was squished to the axis. We got 2 blank templates from CyTOF1 users at SIgN which gave us a range of 0-40K (in increments of 10K) and 0-4K (in increments of 1000). However, in both cases the events were squished to the left on the Event_Length parameter on flowjo. We want to know whether this is a problem of the settings on the CyTOF software, or something that can be resolved with the settings on flowjo, or if it was a consequence of the acquisition speed on the instrument. The pushes looked distinct on the rain display during acquistion, so it's difficult to assume that was the issue, we estimated approximately between 300 and 500events/sec on the different samples, all of which showed the same squished display. Any advice would be appreciated.
Thanks!
Sara