p-STAT5 background
I'm almost ready to go with a panel, but there are a few background issues that I'm concerned about. I've attached a pdf of the dot plots from FlowJo. In the p-STAT5-150Nd channel (purchased from DVS) There is quite a bit of background further up the axis from the real events. I don't think these are real as they don't appear in the FC data collected the same day. The gated MC events correlate quite nicely with the FC events in the frequency and intensity. Possibly the new lot of pSTAT5? Is there some other source for the background? Also CCR7-166Er is showing some background. This is one that I've conjugated with the Maxpar kit to R&Ds clone 150503.
Ian