Thu Jan 23, 2014 11:18 pm by mleipold
Hi Ofir,
I don't have data on that.
However, if you take cells, label them with Ir, and then wash them with water from a dishwashed-bottle a couple times, you should load them with Ba. Then just monitor the Ir channels, Ba138, and 154.
I would recommend doing that, or Rob's metal-minus-one experiment. You won't have spillover into 154(Sm) from 153(Eu) or 155(Gd).
The digestion in HCl or HNO3 is another option, but we've seen some slight differences in contamination/spillovers when run in free metal/liquid mode vs cell mode. This is primarily when looking at antibodies, comparing the impurities in the metal salt stock solution with data from a Kappa-capture-bead experiment with a labeled antibody form that stock. I'm almost positive the metal is present in excess, and while the "good" metal and the "bad" metal *should* enter the polymer chelators at the same ratio with which they're present in solution, I wonder if there's some slight statistical effect in the loading of the polymer when [binding site] << [metal].
That, or we're just under the detection limit for some of the impurities in the antibody-bead experiment.
Mike