CyTOF normalisation using reference PBMCs
I stained thawed "reference" barcoded PMBCs from the same blood donor together with every tested patient sample as a method for future data normalization across different patients and days of measurement. I was wondering if anyone ever used such approach and whether there is already a script how to normalize fcs files across all channels based on reference PBMCs?
I found this paper by Kleinsteuber et al. (https://www.ncbi.nlm.nih.gov/pubmed/?te ... er+corleis) where they used similar approach but only for setting up population gates all across samples, not for normalizing the signal in each channel allowing more complex analysis (such as t-SNE).
I am aware of the beads based normalization by Rachel Fink et al. (https://www.ncbi.nlm.nih.gov/pubmed/23512433). Has anyone tried to modify their method for PBMCs as a standard?
Many thanks for your comments and help!
Ondrej