FAQ  •  Register  •  Login

Flowjo settings

Forum rules
Please be as geeky as possible. Reference, reference, reference.
Also, please note that this is a mixed bag of math-gurus and mathematically challenged, so choose your words wisely :-)



Posts: 17

Joined: Tue Nov 26, 2013 1:55 pm

Post Thu Nov 28, 2013 9:20 am

Flowjo settings

What kind of settings are people using to visualise/analyse CyTOF data on FlowJo? I'm currently using these:
http://iti.stanford.edu/research/docume ... 090512.pdf



Posts: 4300

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Fri Dec 06, 2013 10:16 pm

Re: Flowjo settings

As you might expect from the URL, those are the settings we use at the HIMC :)



Posts: 40

Joined: Mon Dec 02, 2013 3:42 pm

Location: University of Connecticut

Post Wed Dec 11, 2013 9:14 pm

Re: Flowjo settings

I'm on a PC so when Version X isn't working for me I am on version 7.6, here are our settings
FlowJo7.6 settings.png
Display settings used for FlowJo 7.6.5

I went to edit> preferences> data scaling then checked "Auto-transform" and entered those numbers.
I like the number of decades to be greater than 5, additional negative decades should be greater than 0 so we can still visualize the zero values, and the width basis should be as small as possible (-1 is the minimum)

Return to CyTOF data analysis

Who is online

Users browsing this forum: No registered users and 2 guests