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The new Gaussian parameters from Helios

PostPosted: Mon May 08, 2017 1:15 pm
by dahern
Hi,

Recently changed from a CyTOF1 to a the Helios system. I can't find much information on the 4 new parameters added to FCS files by Helios - width, residual, centre and offset. Could anyone point me in the direction of where these are explained or how we can use them to gate out ion fusion events? Is it as simple as just gating the large population as in the attached 2 plots? Do people now use these routinely as part of their gating strategy?

Centre.png
Offset.png


Many thanks,
David.

Re: The new Gaussian parameters from Helios

PostPosted: Mon May 08, 2017 2:34 pm
by RSchackmann
Hi David,
I contacted Fluidigm at the end of April asking about the whitepaper concerning the gaussian parameters. They have been working on it (for the past year at least) and something should appear soon, which I guess means it will be at least another year

,
Ron

Re: The new Gaussian parameters from Helios

PostPosted: Mon May 08, 2017 3:46 pm
by Chowduck
Hi David,

Attached is a document I received from Fluidigm in September of 2016 that describes the Gaussian Discrimination Parameters. I don’t think anyone in our facility is using these parameters for doublet discrimination.

Regards,
-Greg